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WormBook is a comprehensive, open-access collection of original, peer-reviewed chapters covering topics related to the biology of Caenorhabditis elegans and other nematodes. Wormbook also contains: WormMethods, a collection of protocols for nematode researchers; WormHistory, personal perspectives on C. elegans research; and the Worm Breeder's Gazette, an informal, non-refereed, biannual newsletter for the interchange of ideas and information related to C. elegans and other nematodes.
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C. elegans


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News and Notes
June 26 2014
Mass spectrometry chapter available in WormMethods
Mass spectrometry-based shotgun proteomic analysis of C. elegans protein complexes, by Bryan Fonslow, James Moresco, Patricia Tu, Antti Aalto, Amy Pasquinelli, Andrew Dillin and John Yates, has been published in the Cell and molecular biology section of WormMehtods. This chapter describes the use of mass spectrometry-based shotgun proteomics coupled with co-immunoprecipitation to discover new interactions among proteins.
May 9 2014
Endogenous RNAi chapter added
Endogenous RNAi pathways in C. elegans by Allison Billi, Sylvia Fischer, and John Kim, has been published in the Molecular biology section. This chapter reviews current knowledge of endogenous small interfering RNAs and piwi-interacting RNAs.
May 5 2014
Nematode isolation chapter updated in WormMethods
Isolation of C. elegans and related nematodes, by Antoine Barrière and Marie-Anne Félix, has been updated in the Methods in species other than C. elegans section of WormMethods. In addition to providing information on isolation, culture, and freezing conditions, the updated chapter adds information on field collection, and expands information on species identification. The numbering and naming of Caenorhabditis species is described and a list of available collections is provided.
May 2 2014
Links missing from WormBook chapters
In WormBook chapters published between December of 2013 and the present, the links to WormBase for protein names are missing. We apologize for this inconvenience and are working to resolve this problem as soon as possible.
April 28 2014
Updated chapter added to Cell Biology
C. elegans as a model for membrane traffic , by Ken Sato, Anne Norris, Miyuki Sato, and Barth Grant has been published in the Cell biology section. This chapter updates Intracellular trafficking , by Barth Grant and Miyuki Sato. The new chapter summarizes progress in understanding membrane trafficking mechanisms, and focuses mainly on work done in non-neuronal cell-types, especially the germline, early embryo, coelomocytes, and intestine.
April 21 2014
Meeting announcement
Evolutionary Biology of Caenorhabditis and other Nematodes will be held June 14-17, 2014, at the Wellcome Trust Genome Camps, Hinxton, Cambridge, UK. Abstract and Bursary deadline is May 2, Registration deadline is May 16.
April 8 2014
Sperm motility chapter updated
Nematode sperm motility, by Harold Smith, has been published in the Cell biology section of WormBook. This chapter updates the information presented in Sperm motility and MSP and includes expanded discussion on the acquisition, directionality, force generation and guidance of sperm motility.
April 4 2014
New chapter added to WormMethods
Modern techniques for the analysis of chromatin and nuclear organization in C. elegans , by Peter Askjaer, Sevinç Ercan, and Peter Meister, has been published in the Cell and molecular biology section of WormMethods. This chapter provides detailed methods for using ChIP and DamID to identify binding sites for chromatin-associated proteins, methods for ChIP and DamID quantification, and methods for live chromatin imaging.
March 26 2014
New issue of The Worm Breeder's Gazette now available
Volume 20, No. 1 of The Worm Breeder's Gazette has been published. Among the articles are descriptions of improved techniques for mutant identification via whole genome sequencing, culturing C. elegans cells, and preserving histological samples. Meeting and new lab announcements are included along with worm art and humor.
February 24 2014
Thermotaxis methods published in WormBook
Thermotaxis navigation behavior, by Miriam Goodwin et al., has been added to the Behavioral assays section of WormMethods. This chapter gives multiple protocols to assay the navigation of C. elegans hermaphrodites on spatial thermal gradients. A method to assay avoidance of noxious temperatures is also included.
February 19 2014
WormBook congratulates Scott Emmons and colleagues
The Connectome of a Decision-Making Neural Network, by Travis A. Jarrell, Yi Wang, Adam E. Bloniarz, Christopher A. Brittin, Meng Xu, J. Nichol Thomson, Donna G. Albertson, David H. Hall, and Scott Emmons, has received the Newcomb Cleveland Prize, awarded to the most outstanding paper published in Science in the year June 2012 to May 2013. Cited as a tour de force among many competitive entries by Science editor-in-chief Marcia McNutt, this paper presents the wiring diagram of the posterior nervous system of the C. elegans adult male, providing a step toward understanding the connection between neural activity and behavior.
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